DS 29190 PDF

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Terms and conditions apply. Thus, caveolin may organize the formation of caveolae microdomains and orchestrate caveolae-related signaling events. Watch list is full. Email to friends Share on Facebook – opens in a new window or tab Share on Twitter – opens in a new window or tab Share on Pinterest – opens in a new window or tab Add to Watch list.

Email to friends Share on Facebook – opens in a new window or tab Share on Twitter – opens in a new window or tab Share on Pinterest – opens in a new window or tab. These experiments suggest that caveolin may also interact with Src family tyrosine kinases. Caveolin peptides were designed based on the protein sequence of the N-terminal domain of canine caveolin.

These results imply that the interaction of caveolin with Src family kinases may serve to negatively regulate their functional activity. Briefly, phosphorylated protein bands were digitized by high resolution optical scanning; volumetric integration of signal intensities was carried out using ImageQuant Software Fast Scan; Molecular Dynamics.

Thus, in vitro auto-phosphorylation of c-Src serves as a measure of its auto-activation. Cell Culture Insect Sf21 cells were provided by Dr.

Pdyn (prodynorphin) | Gene Report | BioGPS

291190 Submit a Letter to the Editor. Caveolin, a kDa integral membrane protein 78is a major structural and regulatory component of caveolae membranes 7910 The effects of peptide 1 A and peptide 2 B are shown. To test the generality of this phenomenon, we next assessed the effect of caveolin peptides on the auto-phosphorylation of a related Src family tyrosine kinase, Fyn. To date, a total of nine distinct members of the Src family of tyrosine kinases have been identified and cloned Two general classes of tyrosine kinases have been defined: Sign in for checkout Check out as guest.


Cell 6— If the item comes direct from a manufacturer, it may be delivered in non-retail packaging, such as a plain or unprinted box or plastic bag. In vivo effect of the full-length caveolin molecule on the auto-activation of c-Src tyrosine kinase revealed by co-expression of caveolin and c-Src in T cells.

Frequently bought together Feedback on our suggestions – Frequently bought together. Please enter a number less than or equal to 2. Construction and Purification of Glutathione S-Transferase-Caveolin Fusion Proteins The construction, expression, and purification of GST-caveolin 1 fusion proteins were as we described previously 2425 For example, Shoelson and colleagues have shown that high concentrations of peptide substrates inhibited the autophosphorylation of c-Src kinase International postage and import charges paid to Pitney Bowes Inc.

Caveolae are small bulb-shaped invaginations located at or near the cell surface 1. Caveolin-derived Synthetic Peptides Caveolin peptides were designed based on the protein sequence 2190 the N-terminal domain xs canine caveolin.

This amount is subject to change until you make payment. Learn More – opens in a new window or tab Any international postage and import charges are paid in part to Pitney Bowes Inc.

It should be noted that this band was not detectable in uninfected cells or in dd infected with vector alone not shown. Auto-activation of c-Src occurs through auto-phosphorylation of tyrosine In addition, v-Src failed to form a stable complex with caveolin under these binding conditions.

Using an established in vitro binding assay, we find that caveolin interacts with wild-type Src c-Src but does not form a stable complex with mutationally activated Src v-Src. Get an immediate offer. Both classes of tyrosine kinases can function as transforming oncogenes when mutationally activated 50 The cDNA for canine caveolin was as described previously Compatibility information isn’t available for this item. As such, caveolin has served as a marker protein for the organelle 1213 However, in both reports, the investigators used much higher concentrations of peptides, such as 0.


This suggests that caveolin residues are most critical for interaction with c-Src. One possibility is that this caveolin-mediated inhibition is related to tyrosine phosphorylation of caveolin or caveolin-derived peptides, resulting in a form of competitive substrate inhibition of Src family kinases.

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Issues regarding compatibility should be resolved before purchasing. Defining a region of caveolin that contains c-Src binding activity. We do not yet know the mechanism by which caveolin-derived peptides inhibit Src family kinases.


All other reagents were as detailed previously 27 Report item – opens in a new window or tab. Co-expression with caveolin dramatically suppressed the tyrosine kinase activity of c-Src as measured via an immune complex kinase assay.

Wild-type and mutant caveolin peptides detailed in A were examined for their effect on the auto-phosphorylation of purified recombinant c-Src kinase in vitro. For additional information, see the Global Shipping Programme terms and conditions – opens in a new window or tab This amount includes applicable customs duties, taxes, brokerage and other fees. See compatible vehicles Quantity:

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